Composite

Part:BBa_K3789011:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-28)


SOD enzyme secretion system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1023
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1023
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1023
    Illegal NgoMIV site found at 1646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pGK1 promoter can efficiently drive high levels of gene expression. Since the superoxide dismutase 1 enzyme needs to be released in the brewing environment to be effective, we choose the common secretion signal sequence “mating factor alpha prepro-leader (MF alpha)” to make the enzyme secretions become possible. Due to these reasons, the system can provide a stable secretable SOD expression.


Source

pGK1 promoter, Mating factor alpha prepro-leader (MF alpha), and the SOD1 coding sequence come from Saccharomyces cerevisiae S288c. HA-tag sequence comes from the human influenza virus.

References